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Proximal cell K+ activity: technical problems and dependence on plasma K+ concentration.

J Teulon, T Anagnostopoulos

    The American Journal of Physiology
    |July 1, 1982
    PubMed
    Summary
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    Intracellular potassium activity in Necturus kidney proximal tubules was measured using microelectrodes. Results show active potassium pumping into cells and effective homeostatic mechanisms maintaining stable intracellular potassium levels despite external changes.

    Area of Science:

    • Nephrology
    • Cell Physiology
    • Biophysics

    Background:

    • Intracellular potassium activity (alpha Ki) is crucial for kidney tubule cell function.
    • Accurate measurement of alpha Ki in vivo presents methodological challenges.

    Purpose of the Study:

    • To measure intracellular K+ activity (alpha Ki) in Necturus kidney proximal tubular cells.
    • To investigate the impact of peritubular potassium concentration (CpK) on alpha Ki.
    • To assess the mechanisms of potassium homeostasis in these cells.

    Main Methods:

    • Utilized K+-selective liquid ion-exchanger double-barreled microelectrodes for in vivo measurements.
    • Addressed methodological issues including leaky impalements and accurate alpha Ki assessment.
    • Manipulated peritubular capillary potassium concentration (CpK) using double-barreled micropipettes.

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    Main Results:

    • Controlled alpha Ki averaged 57.9 +/- 8.5 mM with a basolateral membrane potential of -70.4 +/- 5.8 mV.
    • Increasing CpK from 3.0 to 30.0 mM raised alpha Ki by 7.0 +/- 1.5 mM.
    • Decreasing CpK to 0.3 mM lowered alpha Ki by 9.2 +/- 2.9 mM.

    Conclusions:

    • Steady-state K+ distribution suggests active pumping of potassium into proximal tubular cells.
    • The stability of alpha Ki during CpK variations highlights effective cellular homeostasis, involving passive mechanisms.