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Related Experiment Videos

Macrophage aggregation factor: some properties.

P Badenoch-Jones

    Immunology
    |July 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Macrophage aggregation factor (MAgF) activity was found in spleen cell cultures and was heat-stable. This factor enhances both cell-substrate and cell-cell adhesion.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Lymphokines are signaling molecules crucial for immune responses.
    • Macrophage aggregation factor (MAgF) is a lymphokine implicated in immune cell interactions.

    Purpose of the Study:

    • To further investigate the properties and functions of macrophage aggregation factor (MAgF).

    Main Methods:

    • Spleen cell culture supernatants were analyzed for MAgF activity.
    • MAgF was subjected to dialysis, heat treatment, enzymatic digestion (alpha-chymotrypsin, trypsin, neuraminidase), protein precipitation, periodate treatment, and Concanavalin A-Sepharose binding.
    • Polyacrylamide gel electrophoresis (PAGE) was used for migration analysis.
    • Cellular aggregation was measured via batch centrifugation assays.

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    Main Results:

    • MAgF activity was consistently detected in test supernatants but not controls.
    • Activity increased after dialysis and was heat-stable.
    • MAgF was inactivated by alpha-chymotrypsin, partially by trypsin, and unaffected by neuraminidase.
    • Purification was achieved via perchloric acid precipitation.
    • Minimal loss of activity occurred after periodate treatment; no Concanavalin A binding was observed.
    • PAGE indicated MAgF migrates cathodally to albumin.
    • MAgF promotes both cell-substrate and cell-cell adhesion.

    Conclusions:

    • MAgF is a heat-stable lymphokine produced by spleen cells.
    • Its properties suggest a proteinaceous nature, with specific enzymatic sensitivities.
    • MAgF plays a significant role in mediating macrophage adhesion processes.