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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection
Published on: July 5, 2013
Parotid acinar cells: ionic dependence of isoprenaline-evoked membrane potential changes
Abstract:
The effect of microionophoretic application of isoprenaline on membrane potential and resistance of mouse parotid acinar cells was investigated. For measurements of membrane resistance and the isoprenaline equilibrium potential (Eiso), two microelectrodes were inserted into neighbouring communicating cells. Passing direct current through one of these electrodes, the resting potential could be set at desired levels and Eiso was determined by plotting the relation between the size of the isoprenaline-evoked potential change and the resting potential. Simple depolarizations were found at relatively high resting potentials, while biphasic potential changes in response to isoprenaline (hyperpolarization followed by depolarization) were observed at low resting potentials. Both depolarizing and hyperpolarizing responses to isoprenaline were accompanied by a reduction of membrane resistance. The isoprenaline equilibrium potential in the initial phase of the response was about -53 mV, but had a value of about -24mV in the delayed phase. The initial isoprenaline-evoked potential change was sensitive to alterations in extracellular Na, K and Cl concentrations. The delayed depolarizing response to isoprenaline was markedly reduced by replacing extracellular Na by Tris or extracellular Cl by SO4. These results indicate that isoprenaline opens up conductance pathways permeable to Na and K.
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