E-rosette formation with SRBC and changes in cAMP level of hog lymphocytes--a study of certain affecting factors

A further study of cAMP change in porcine lymphocytes after E-rosette formation is presented in the present report using peripheral blood lymphocytes of pig mixed with different concentrations of SRBC membrane as well as SRBC treated with different enzymes for modification of the rosette formation. It has been found that cAMP enhancement in lymphocyte follows the increase in the concentration of SRBC membrane used, and that a similar relation appeared between modification of cAMP and enzyme-treated SRBC in the rosette formation. It has also been found that after rosetting with SRBC, cAMP level of lymphocytes began to rise within 1 min, reached its peak at 10 min and then returned to control level in about 8 hr.