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Conjugative R plasmids in Streptococcus faecium (group D).

C Le Bouguenec, T Horodniceanu

    Antimicrobial Agents and Chemotherapy
    |May 1, 1982
    PubMed
    Summary
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    This study found that Streptococcus faecium strains exhibit resistance to multiple antibiotics, including tetracycline and macrolides. Some strains transfer these antibiotic resistance genes via plasmids, indicating potential for spread.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Antibiotic resistance in bacteria is a growing global health concern.
    • Streptococcus faecium is an opportunistic pathogen known to acquire and disseminate resistance genes.

    Purpose of the Study:

    • To investigate the antibiotic resistance profiles of Streptococcus faecium isolates.
    • To characterize the role of plasmids in the transfer of antibiotic resistance markers within S. faecium.

    Main Methods:

    • Antibiotic susceptibility testing was performed on Streptococcus faecium isolates.
    • Conjugation experiments were conducted to assess the transfer of resistance markers.
    • Plasmid DNA was isolated and analyzed using molecular techniques, including restriction enzyme digestion.

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    Main Results:

    • Ten S. faecium isolates displayed resistance to multiple antibiotics, including penicillin, tetracycline, macrolides, streptomycin, and kanamycin.
    • Six resistant strains transferred resistance markers (excluding penicillin resistance) via conjugation at low frequencies.
    • Analysis revealed the presence of multiple plasmids in wild-type strains, with some transconjugants harboring plasmids identical or different in size to donor plasmids, suggesting molecular events during transfer.
    • HindIII restriction enzyme fingerprinting differentiated S. faecium resistance plasmids from those of other streptococcal strains.

    Conclusions:

    • Streptococcus faecium possesses diverse antibiotic resistance mechanisms, often mediated by plasmids.
    • The conjugative transfer of resistance plasmids, potentially involving molecular alterations, contributes to the dissemination of antibiotic resistance in S. faecium.
    • Distinct plasmid profiles suggest strain-specific resistance determinants in S. faecium.