Induction of a chemotactic factor from human neutrophils by diverse crystals

The purpose of this study was to isolate and compare the chemotactic factor generated by human neutrophils after phagocytosis of three structurally different crystals. An apparently identical chemotactic factor for neutrophils was isolated from the granular fraction of human neutrophils allowed to phagocytose MSU, CPPD, or amorphous DC. The isolated chemotactic factors migrated identically on SDS polyacrylamide gel electrophoresis, with a relative motility of 8.05, and competed with MSU-induced chemotactic factor for binding sites on the human neutrophils in an identical fashion. The data support the concept that the generation of the chemotactic factor represents a general response of the neutrophil to the phagocytosis of foreign particles.