Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An immunoglobulin VH pseudogene.

H Huang, S Crews, L Hood

    Journal of Molecular and Applied Genetics
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Researchers discovered a non-functional VH gene segment, a pseudogene, within the T15 group. This finding highlights the frequent occurrence of pseudogenes in multigene families like those involved in antibody formation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Vasomotor rhinitis following endoscopic sinus surgery: 5 cases report].

    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology·2003
    Same author

    [Correlation of p16 mutation and biological behavior in Chinese laryngeal cancer].

    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology·2003
    Same author

    [Primary restoration for congenital malformation of external and middle ear].

    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology·2003
    Same author

    [Diagnostic value of computer-based endoscopy system].

    Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology·2003
    Same author

    [Guided tissue regeneration with Biomesh membrane for Class II furcation defects].

    Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2003
    Same author

    Comparative study on the immunogenicity between Hsp70 DNA vaccine and Hsp65 DNA vaccine in human Mycobacterium tuberculosis.

    Journal of Tongji Medical University = Tong ji yi ke da xue xue bao·2003
    Same journal

    Plant tRNA genes: putative soybean genes for tRNAasp and tRNAmet.

    Journal of molecular and applied genetics·1985
    Same journal

    Structure and expression of two beta genes in a beta thalassemia homozygote.

    Journal of molecular and applied genetics·1985
    Same journal

    Identification of the promoter of the Pseudomonas gene coding for carboxypeptidase G2.

    Journal of molecular and applied genetics·1985
    Same journal

    Purification of hybrid beta-galactosidase proteins encoded by phi X174 E phi lacZ and Escherichia coli prlA phi lacZ: a general method for the isolation of lacZ fusion polypeptides produced in low amounts.

    Journal of molecular and applied genetics·1985
    Same journal

    Comparison of the transient late region expression of SV40 DNA and SV40-based shuttle vectors: development of a new shuttle vector that is efficiently expressed.

    Journal of molecular and applied genetics·1985
    Same journal

    A chlorophyll a/b-protein encoded by a gene containing an intron with characteristics of a transposable element.

    Journal of molecular and applied genetics·1985
    See all related articles

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • The T15 group of VH gene segments is crucial for the mouse immune response to phosphorylcholine.
    • Understanding gene regulation and mutation is key to comprehending immune system function.

    Purpose of the Study:

    • To identify and characterize novel VH gene segments within the T15 group.
    • To investigate the mechanisms leading to the inactivation of antibody gene segments.

    Main Methods:

    • Sequence analysis of VH gene segments.
    • Identification of mutations affecting gene expression.
    • Homology comparison with known VH gene segments.

    Main Results:

    • A novel pseudogene within the T15 VH gene group was identified.

    Related Experiment Videos

  • Three specific mutations (in-frame stop codon, frameshift insertion, altered recognition elements) were found to inactivate the gene segment.
  • The pseudogene exhibits high homology (88-96%) to functional T15 VH genes, suggesting recent origin.

    • Conclusions:

    • Pseudogene formation is a likely and frequent event in multigene families, particularly antibody gene families.
    • The complex processes of DNA rearrangement and RNA splicing in antibody gene expression provide multiple pathways for pseudogene creation.
    • Pseudogenes may eventually be deleted from the gene family, contributing to evolutionary dynamics.