Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in mouse oocyte membrane potential and permeability during meiotic maturation.

R D Powers

    The Journal of Experimental Zoology
    |July 1, 1982
    PubMed
    Summary

    Mouse oocytes undergo spontaneous meiotic maturation, involving significant changes in cell membrane potential. Reduced potassium permeability is identified as the primary driver of membrane depolarization during this process.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Echinococcosis in a drill baboon.

    Journal of the American Veterinary Medical Association·2012
    Same author

    Serum prolactin and TSH in an in vitro fertilization population: is there a link between fertilization and thyroid function?

    Journal of assisted reproduction and genetics·2003
    Same author

    Emergency department use by adult medicaid patients after implementation of managed care.

    Academic emergency medicine : official journal of the Society for Academic Emergency Medicine·2000
    Same author

    The interpretation and use of statistics in assisted reproductive technologies.

    Obstetrics and gynecology clinics of North America·2000
    Same author

    Variation of docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation: implications for sperm lipoperoxidative damage.

    Molecular reproduction and development·2000
    Same author

    Is the mouse a clinically relevant model for human fertilization failures?

    Human reproduction (Oxford, England)·1999

    Area of Science:

    • Cell Biology
    • Reproductive Biology
    • Membrane Physiology

    Background:

    • Mouse oocytes mature spontaneously in vitro, a process involving critical cellular changes.
    • Understanding the electrophysiological events during oocyte maturation is crucial for reproductive science.

    Purpose of the Study:

    • To investigate the changes in cell surface membrane potential during mouse oocyte meiotic maturation.
    • To determine the ionic basis of membrane potential changes and their relationship with germinal vesicle breakdown (GVBD).

    Main Methods:

    • Measurement of cell surface membrane potential at different meiotic stages (GV, GVBD, PB).
    • Radioisotope tracer flux experiments to quantify potassium (K) transport parameters.
    • Pharmacological manipulation using dibutyryl cyclic AMP to block GVBD.

    Related Experiment Videos

    Main Results:

    • Membrane potential depolarized from -46 mV (GV stage) to -17 mV (PB stage).
    • Potassium (K) efflux rate coefficient and permeability were determined at the GV stage.
    • A decrease in K permeability was linked to membrane depolarization.
    • Blocking GVBD with dibutyryl cyclic AMP prevented depolarization, suggesting a linkage.
    • Oocyte depolarization via high K+ medium did not induce GVBD.

    Conclusions:

    • Membrane potential depolarization during mouse oocyte maturation is primarily due to decreased K+ permeability.
    • While linked to GVBD, membrane depolarization alone is insufficient to trigger GVBD.