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Related Experiment Videos

A transforming gene present in human sarcoma cell lines.

C J Marshall, A Hall, R A Weiss

    Nature
    |September 9, 1982
    PubMed
    Summary

    Researchers identified a novel transforming gene in human sarcomas. This gene, found in fibrosarcoma and embryonal rhabdomyosarcoma, differs from previously identified oncogenes in other human tumors and lacks homology to known retroviral oncogenes.

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    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • DNA transfection into NIH/3T3 cells identifies transforming sequences in human tumors.
    • Transforming activity has been detected in various tumor types, including carcinomas and hematological malignancies.
    • Previously identified transforming sequences varied by tumor type, with some overlap between colon and lung carcinomas.

    Purpose of the Study:

    • To investigate the presence and nature of transforming genes in human sarcoma cell lines.
    • To determine if the transforming gene in sarcomas is similar to those found in other human tumors.
    • To assess the homology of the sarcoma-derived transforming gene to known retroviral oncogenes.

    Main Methods:

    • DNA transfection of NIH/3T3 cells.
    • Isolation and analysis of DNA from human fibrosarcoma and embryonal rhabdomyosarcoma cell lines.
    • Southern blot analysis to detect homology to retroviral oncogenes.

    Main Results:

    • DNAs from two distinct human sarcoma cell lines (fibrosarcoma and embryonal rhabdomyosarcoma) exhibited transforming activity.
    • The transforming gene identified in both sarcomas was identical to each other.
    • This sarcoma-specific transforming gene showed no detectable homology to eight retroviral oncogenes tested.

    Conclusions:

    • Human sarcomas harbor a specific transforming gene distinct from those found in other tumor types.
    • The identified transforming gene represents a novel oncogene not previously characterized.
    • Further research is warranted to elucidate the function and mechanism of this novel sarcoma-associated transforming gene.

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