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2-chloroadenosine increases calcium mobilization from mouse calvaria in vitro
Acta Endocrinologica
|June 1, 1982
Summary
2-Chloroadenosine stimulates bone resorption in mouse calvarial bones, indicating a cell-mediated process. While it transiently increases cyclic AMP (cAMP) formation, the initial bone resorption effect may not be cAMP-dependent.
Area of Science:
- Biochemistry
- Cell Biology
- Bone Biology
Background:
- Adenosine signaling plays a role in bone metabolism.
- Understanding the mechanisms of bone resorption is crucial for treating bone diseases.
Purpose of the Study:
- To investigate the effect of 2-chloroadenosine on bone resorption and cyclic AMP (cAMP) formation in murine calvarial bones.
- To determine if the effects of 2-chloroadenosine on bone are cell-mediated.
Main Methods:
- In vitro culture of murine calvarial bones.
- Measurement of 45Ca release to assess bone resorption.
- Measurement of cyclic AMP (cAMP) accumulation.
- Use of 8-Bromo cyclic AMP (cAMP) to investigate the role of cAMP.
Main Results:
- 2-Chloroadenosine significantly increased 45Ca release from cultured bones, indicating enhanced bone resorption.
- The effect was cell-mediated, as dead bones showed no response.
- A transient stimulation of cyclic AMP (cAMP) formation was observed.
- The dose-response for 45Ca release showed a decline at higher concentrations.
- 8-Bromo cyclic AMP (cAMP) inhibited 45Ca release, suggesting the initial effect of 2-chloroadenosine might not be cAMP-dependent.
Conclusions:
- 2-Chloroadenosine stimulates bone resorption in a cell-mediated manner.
- The initial bone resorption stimulated by 2-chloroadenosine may not be directly dependent on cyclic AMP (cAMP).
- Bone tissue can form adenosine, as indicated by increased inosine levels during culture.