Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclic AMP mediated morphological changes in a hybrid cell line.

R Aston, S R Ayad

    Anticancer Research
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Accuracy of routine data on MMR vaccination coverage and validity of parental recall of vaccination.

    Communicable disease and public health·2003
    Same author

    Susceptibility of young offenders to measles and rubella: an antibody prevalence study using oral fluid samples.

    Communicable disease and public health·2003
    Same author

    Vaccination in older people.

    Nursing standard (Royal College of Nursing (Great Britain) : 1987)·2002
    Same author

    Prevention of pneumococcal disease in young children.

    Nursing standard (Royal College of Nursing (Great Britain) : 1987)·2002
    Same author

    Measles elimination: a mass immunization campaign in Romania.

    American journal of public health·2001
    Same author

    Acceptability of health status questionnaire screening and vaccination among Kosovar refugees.

    Communicable disease and public health·2000

    A hybrid cell line (PCM 3) transforms from flat to spherical cells when intracellular cyclic adenosine monophosphate (cAMP) levels rise. This dose-dependent, energy-requiring shape change is influenced by phosphodiesterase inhibitors and serum factors.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Cancer Research

    Background:

    • Hybrid cell lines offer unique models for studying cellular processes.
    • Cyclic adenosine monophosphate (cAMP) is a crucial second messenger involved in various cellular functions.
    • Understanding cell morphology changes is vital for cancer research and drug development.

    Purpose of the Study:

    • To investigate the morphological transformation of the PCM 3 hybrid cell line.
    • To elucidate the role of intracellular cAMP in inducing cell rounding.
    • To identify factors that modulate cAMP-induced cell shape changes.

    Main Methods:

    • Utilized the PCM 3 hybrid cell line, a fusion of Chinese hamster fibroblasts and a malignant lymphoma.
    • Administered agents that elevate intracellular cAMP and phosphodiesterase inhibitors.

    Related Experiment Videos

  • Assessed cell morphology, adhesion, and energy dependence of the transformation.
  • Investigated changes in plasma membrane proteins and the effect of serum factors.

    • Main Results:

    • PCM 3 cells convert from a flat, spindle-shaped monolayer to spherical cells upon elevation of intracellular cAMP.
    • This dose-dependent morphological change is not associated with a loss of cell adhesion.
    • The cell rounding is energy-dependent and enhanced by phosphodiesterase inhibitors.
    • While similar to cytochalasin B-induced changes, plasma membrane protein alterations differ.
    • Specific serum factors were found to inhibit the cAMP-induced cell rounding.

    Conclusions:

    • Intracellular cAMP levels significantly regulate the morphology of PCM 3 hybrid cells.
    • The cAMP-induced cell rounding is an active, energy-dependent process.
    • Serum factors can counteract the effects of elevated cAMP on cell shape, suggesting complex regulatory mechanisms.