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Related Concept Videos

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Nucleotide Excision Repair

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Translesion DNA Polymerases02:10

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Homologous Recombination02:31

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Related Experiment Video

Updated: Jul 18, 2026

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

T4 endonuclease VII cleaves holliday structures.

K Mizuuchi, B Kemper, J Hays

    Cell
    |June 1, 1982
    PubMed
    Summary

    T4 endonuclease VII cleaves Holliday structures, resolving DNA replication intermediates. Its action with DNA ligase produces genetically sensible products, clarifying its role in phage T4 DNA metabolism.

    Area of Science:

    • Molecular Biology
    • Enzymology
    • DNA Replication and Repair

    Background:

    • Holliday structures are key intermediates in DNA recombination and repair.
    • T4 endonuclease VII is involved in the DNA metabolism of bacteriophage T4.

    Purpose of the Study:

    • To elucidate the mechanism by which T4 endonuclease VII resolves Holliday structures.
    • To understand the role of T4 endonuclease VII in generating genetically sensible DNA products.

    Main Methods:

    • In vitro cleavage assays using purified T4 endonuclease VII.
    • Analysis of DNA products generated by endonuclease VII activity.

    Main Results:

    • T4 endonuclease VII specifically cleaves both strands of Holliday structures at or near the branch point.

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    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
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    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

    Published on: May 24, 2017

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    Last Updated: Jul 18, 2026

    Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
    09:40

    Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

    Published on: September 23, 2011

    A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
    11:25

    A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

    Published on: March 18, 2017

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
    10:59

    Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

    Published on: May 24, 2017

  • Cleavage generates two unbranched DNA duplexes, each with a single-strand break.
  • The strand breaks occur at identical positions within the nucleotide sequence on each strand.

    • Conclusions:

    • T4 endonuclease VII acts on the branch point of duplex DNA, resolving Holliday structures.
    • The combined action of T4 endonuclease VII and DNA ligase can repair strand breaks, yielding functional DNA.
    • These findings explain the enzyme's function in phage T4 DNA metabolism and identify it as the first enzyme with specific branch point activity.