Phenotypic differences between tumor cells derived from different stages of neoplastic growth

Under conditions employed in our laboratory, tumors which are induced by avian sarcoma virus (ASV) usually grow progressively for several weeks and then regress. In order to further understand the basis for tumor regression in this model, we compared avian sarcoma cells which were cultured from tumors at different stages of development in terms of various phenotypic properties. The results indicate that tumor cells which are derived from progressively-growing sarcomas are rapidly growing, produce large quantities of the enzyme plasminogen activator, and have much in common generally with chicken embryo fibroblast (CEF) cells that have been transformed by ASV. In contrast, tumor cells that are obtained from regressors have elevated levels of hexose transport, grow very slowly, are greatly enlarged and display properties that are characteristic of senescent cells in culture.