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Serological analysis of cell surface antigens on methylcholanthrene-induced mouse sarcomas with antisera produced
Abstract:
Out of 9 cell lines from methylcholanthrene-induced fibrosarcomas in BALB/c, 4 lines without expression of murine leukemia virus (MuLV) were selected for immunization of three different F1 hybrids with mitomycin C-treated in vivo sarcoma cells. Antibody activity was tested by immune adherence (IA) and protein A (PA) assays after the 8th immunization. Two sarcomas, CMC-11 and CMC-12, produced antibodies detectable by IA in (BALB/c x C3H/He)F1, but no antibodies were detected by PA. (BALB/c x C3H/He)F1 anti-CMC-11 was tested against CMC-11 and three other MuLV- sarcomas as well as against the skin fibroblast (C-11) from a BALB/c mouse, in which the primary CMC-11 sarcoma was induced. CMC-11 line reacted up to 1/80, but other lines were weakly positive or negative. Absorption analysis showed that the antigen defined by anti-CMC-11 was present on CMC-11 as well as on many other sarcoma cell lines, but not on BALB/c thymocytes and spleen cells, nor on leukemia and myeloma cells. Among 5 fibroblast cultures tested, C-14 showed complete absorption. It seems significant that only C-14 produced tumor nodules in BALB/c mice, in contrast to 4 other fibroblast cultures. This suggested that this antibody was directed against antigens present on transformed fibroblasts and sarcoma cells. The specificity of (BALB/c x C3H/He)F1 anti-CMC-12 serum indicated that this antiserum also seemed to detect transformation-related antigens, although it showed a broader specificity with regard to the tissue representation detected.
Insights
Researchers developed antibodies targeting specific antigens on fibrosarcoma cells. These antibodies show potential for detecting transformed fibroblasts and sarcoma cells, aiding cancer research.
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Murine leukemia virus (MuLV)-negative fibrosarcoma cell lines were established from methylcholanthrene-induced sarcomas in BALB/c mice.
- These cell lines were used to generate antibodies in F1 hybrid mice.
Purpose of the Study:
- To develop and characterize antibodies against tumor-associated antigens on fibrosarcoma cells.
- To investigate the specificity of these antibodies for transformed cells and sarcomas.
Main Methods:
- Immunization of F1 hybrid mice with mitomycin C-treated fibrosarcoma cells.
- Antibody activity was assessed using immune adherence (IA) and protein A (PA) assays.
- Antigen specificity was determined through absorption analysis with various cell types.
Main Results:
- Antibodies generated against CMC-11 and CMC-12 fibrosarcomas were detected by IA.
- The anti-CMC-11 antibody reacted strongly with the immunizing sarcoma and other sarcoma cell lines, but not with normal lymphocytes or leukemia/myeloma cells.
- Absorption analysis indicated the antigen is present on transformed fibroblasts and sarcoma cells, with anti-CMC-12 showing broader specificity.
Conclusions:
- The generated antibodies are directed against transformation-related antigens present on fibrosarcoma cells.
- These antibodies may serve as valuable tools for identifying and studying tumor-associated antigens in cancer research.