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HLA-D typing using Epstein-Barr virus-induced lymphoblastoid cell lines.
The Japanese Journal of Experimental Medicine
|April 1, 1982
Summary
Epstein-Barr virus-induced lymphoblastoid cell lines (EBV-LCL) can substitute for homozygous typing cells (HTC) in HLA-D typing. This method addresses the shortage of HTC, enabling reliable HLA-D typing using EBV-LCL.
Area of Science:
- Immunogenetics
- Cell Biology
Background:
- Human Leukocyte Antigen (HLA) typing is crucial for transplantation.
- A shortage of homozygous typing cells (HTC) limits HLA-D typing.
- Epstein-Barr virus-induced lymphoblastoid cell lines (EBV-LCL) are potential alternatives.
Purpose of the Study:
- To evaluate EBV-LCL as a substitute for HTC in HLA-D typing.
- To establish a reliable method for HLA-D typing using EBV-LCL.
Main Methods:
- Established EBV-LCL from homozygous typing cells (HTC-EBV-LCL).
- Performed one-way mixed lymphocyte reactions (MLR) using EBV-LCL as stimulators.
- Optimized stimulator: responder ratios (1:10) for MLR.
Main Results:
- Autologous MLR with EBV-LCL showed low double normalized values (DNV) at a 1:10 ratio.
- Homologous combinations were distinguishable from autologous ones.
- Repeat MLR using EBV-LCL derived from HTC showed consistent low DNV.
Conclusions:
- HTC-EBV-LCL can effectively substitute for normal HTC in HLA-D typing.
- This method overcomes the scarcity of HTC, facilitating HLA-D typing.
- The optimized MLR protocol ensures reliable typing results.