Variety of determinants in an adrenal antigen common to man and some animals

Precipitating adrenal antibodies are specifically associated with the moniliasis-polyendocrinopathy syndrome. This may include several simultaneous autoimmune disorders such as Addison's disease, hypoparathyroidism, thyroiditis, pernicious anaemia and ovarian failure often combined with moniliasis and alopecia. The antibodies react with two adrenal specific antigens present in man and in different animals. One of these antigens designated S-(soluble) antigen is present in all subcellular fractions and the other designated P-(particulate) antigen was found in precipitable form only in the mitochondrial fractions. Rabbits immunized with bovine adrenal homogenate and different subcellular fractions, produced three to four adrenal specific antibodies, which were qualitatively identical. The corresponding antigens were designed as S1-S4, one of which (S4) was strictly specific for bovine tissue. S1-, S2- and S3-antigens were identical in bovine and ovine adrenals, whereas a partial reaction of identity was found between ruminants and the S1- and S2-antigens of all other species tested. The S1- and S2-antigens seem to contain a variety of determinants, some common to all the species studied and some limited to certain species only. Of the four S-antigens only one (S1) in rabbits, was found to contain autoantigenic determinants. Comparative studies with sera from patients with the moniliasis-polyendocrinopathy syndrome indicate, that this molecule is the one with which human and rabbit antisera react.