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Related Experiment Videos

Collagenolytic activity in keratoconus.

U Rehany, M Lahav, S Shoshan

    Annals of Ophthalmology
    |August 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Collagenolytic activity, an enzyme that breaks down collagen, is elevated in keratoconus corneas. This suggests increased collagenase activation may contribute to the development of this corneal disease.

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    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Cell Biology

    Background:

    • Keratoconus is a progressive corneal disease characterized by thinning and irregular protrusion.
    • The exact pathogenesis of keratoconus remains incompletely understood.
    • Collagen degradation is implicated in tissue remodeling and disease progression.

    Purpose of the Study:

    • To investigate collagenolytic activity in corneal tissue from patients with advanced keratoconus.
    • To compare enzyme activity between keratoconus and normal corneas.
    • To explore the potential role of collagenase activation in keratoconus development.

    Main Methods:

    • Corneal buttons from eight keratoconus patients and controls were cultured.
    • Organ culture medium was assayed for collagenolytic activity.

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  • Activity was measured using 14C-labeled collagen and assessed with and without trypsin activation.

    • Main Results:

    • Corneal explants from keratoconus patients exhibited significantly higher collagenolytic activity.
    • Enzyme activity was detected in the culture medium of keratoconic corneas.
    • This activity was enhanced by trypsin activation, indicating the presence of procollagenases.

    Conclusions:

    • Elevated collagenolytic activity is present in advanced keratoconus corneas.
    • Increased collagenase activation may be a key pathogenic factor in keratoconus.
    • Further research into collagenase pathways could reveal therapeutic targets.