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Protein phosphorylation in isolated synaptic junctional structures: changes during development.

P T Kelly

    Brain Research
    |September 9, 1982
    PubMed
    Summary
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    Synaptic junction protein phosphorylation increases significantly during early postnatal development. This maturation of cyclic AMP-stimulated phosphorylation correlates with synapse formation in the brain.

    Area of Science:

    • Neuroscience
    • Developmental Biology
    • Molecular Biology

    Background:

    • Synaptic plasticity and function rely on dynamic protein phosphorylation.
    • Understanding the developmental regulation of protein phosphorylation is crucial for comprehending synapse maturation.

    Purpose of the Study:

    • To investigate endogenous protein phosphorylation in synaptic fractions during postnatal development.
    • To examine the role of cyclic AMP-stimulated phosphorylation in synaptic maturation.

    Main Methods:

    • In vitro phosphorylation assays using synaptic plasma membrane (SPM) and synaptic junction (SJ) fractions.
    • Measurement of protein kinase activity with endogenous and exogenous substrates.
    • Analysis of cyclic AMP-regulated protein phosphorylation.

    Related Experiment Videos

  • Photoaffinity labeling to quantify cyclic AMP-dependent kinase subunits.

    • Main Results:

    • Kinase activity and cyclic AMP-stimulated phosphorylation increased significantly in SPM and SJ fractions between postnatal days 5 and 15.
    • Specific endogenous proteins (73, 70, 65, and 55 kDa) in SJ fractions showed age-dependent phosphorylation changes.
    • Phosphorylation of these proteins resulted from their de novo appearance, not altered kinase-substrate coupling.
    • Regulatory subunits of cyclic AMP-dependent kinases remained stable during development.

    Conclusions:

    • Synaptic junction protein phosphorylation undergoes significant enzymatic maturation during early postnatal development.
    • The observed changes in cyclic AMP-stimulated phosphorylation correlate with synapse development.
    • This maturation process is critical for the functional development of synapses.