Simple sandwich enzyme immunoassay for quantification of mouse mammary tumor virus in mouse milk

We have developed a sandwich enzyme immunoassay in order to measure quantitatively mouse mammary tumor virus (MMTV) in mouse milk. In this assay, the antibody-beta-D-galactosidase complex and antibody-bound silicon rubber pieces pieces as solid phase are used. The assay is able to detect 10 ng/ml of MMTV in the milk sample.