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Cyclic AMP mediated modulation of complement protein production.

D Lappin, K Whaley

    International Journal of Immunopharmacology
    |January 1, 1982
    PubMed
    Summary
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    Cyclic AMP (cAMP) analogues and phosphodiesterase inhibitors reduce monocyte C2 production by impairing protein secretion and increasing degradation. These findings suggest intracellular cAMP levels regulate monocyte protein synthesis.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Monocytes play a crucial role in the immune system, including the production of complement proteins.
    • The regulation of monocyte protein production is complex and involves various signaling pathways.

    Purpose of the Study:

    • To investigate the mechanisms by which cAMP analogues and phosphodiesterase inhibitors affect C2 production by monocytes.
    • To elucidate the role of intracellular cAMP levels in controlling monocyte protein synthesis and secretion.

    Main Methods:

    • Monocytes were cultured and treated with cAMP analogues and phosphodiesterase inhibitors.
    • Pulse labeling studies using 3H-labelled amino acids were performed to assess protein synthesis and secretion.
    • Intracellular degradation of newly synthesized proteins was measured.

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  • Temporal relationships between changes in protein synthesis, secretion, and degradation were analyzed.

    • Main Results:

    • Dibutyryl cAMP (dbcAMP) impaired the secretion of total and individual complement proteins.
    • Increased intracellular degradation of newly synthesized proteins was observed in dbcAMP-treated cultures.
    • Protein synthesis was significantly reduced by day 3 of culture.
    • Protein secretion was impaired early (day 1), while increased degradation was evident by day 2.

    Conclusions:

    • cAMP analogues and phosphodiesterase inhibitors reduce monocyte C2 production.
    • Changes in intracellular cAMP levels appear to act as a second signal in regulating monocyte protein production.
    • The observed effects involve impaired protein secretion, increased intracellular degradation, and reduced protein synthesis.