[Energy metabolism in subcellular fractions of normal and acute ischemic kidneys]

The level of oxidative phosphorylation, activity of phosphofructokinase, fructose-1,6-diphosphate aldolase, ketose-1-phosphate aldolase, glucose-6-phosphatase and lactate dehydrogenase are determined in subcellular fractions in the kidney cortex layer of rabbits which have suffered from acute ischemia (for 15, 30, 60, 120 min). Ischemia inhibits the oxidative processes in mitochondria which is proportional to the duration of the effect. An increase in the activity of glycolytic chain enzymes in microsomes and soluble fraction for 15-30 min of ischemia evidences for a compensation of the energy losses at the expense of glycolysis with short periods of ischemia. Glycolysis is inhibited with a more prolonged effect. It is established that the anti-ischemic protection of the organ viability is to be conducted not only with allo- but also with auto-transplantation of the kidney in case of short acute ischemia.