Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-function relations of the thyrotropin receptor.

B R Smith, P R Buckland

    Ciba Foundation Symposium
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inhibition of HERV-K (HML-2) in amyotrophic lateral sclerosis patients on antiretroviral therapy.

    Journal of the neurological sciences·2021
    Same author

    Detection of first gammaherpesvirus sequences in Central African bats.

    New microbes and new infections·2020
    Same author

    Viral emergence in marine mammals in the North Pacific may be linked to Arctic sea ice reduction.

    Scientific reports·2019
    Same author

    A Health Services Research Agenda for Bariatric Surgery Within the Veterans Health Administration.

    Journal of general internal medicine·2017
    Same author

    Genetic dissection of intraspecific variation in a male-specific sexual trait in Drosophila melanogaster.

    Heredity·2016
    Same author

    Catalase and the production of brain acetaldehyde: a possible mediator of the psychopharmacological effects of ethanol.

    Addiction biology·2016
    Same journal

    Precision agriculture: spatial and temporal variability of environmental quality. General reflections.

    Ciba Foundation symposium·1997
    Same journal

    Uncertainty in hydrogeological modelling.

    Ciba Foundation symposium·1997
    Same journal

    Optimal mapping of site-specific multivariate soil properties.

    Ciba Foundation symposium·1997
    Same journal

    Modelling for precision weed management.

    Ciba Foundation symposium·1997
    Same journal

    GIS support for precision agriculture: problems and possibilities.

    Ciba Foundation symposium·1997
    Same journal

    Spatial sampling.

    Ciba Foundation symposium·1997
    See all related articles

    The TSH receptor, a glycoprotein, binds thyroid-stimulating hormone (TSH) and Graves

    Area of Science:

    • Endocrinology and Immunology
    • Molecular and Cellular Biology

    Background:

    • The thyrotropin (thyroid-stimulating hormone or TSH) receptor is a key membrane protein involved in thyroid hormone regulation.
    • Graves' disease, an autoimmune disorder, is characterized by antibodies that interact with the TSH receptor, leading to hyperthyroidism.

    Purpose of the Study:

    • To characterize the physicochemical properties of the TSH receptor.
    • To investigate the interaction between TSH receptor antibodies and the TSH receptor in Graves' disease.
    • To elucidate the relationship between TSH and antibody binding sites on the TSH receptor.

    Main Methods:

    • Gel filtration to determine molecular mass.
    • Isoelectric focusing to determine isoelectric point.
    • Chemical, enzymic, and affinity probes for structural analysis.

    Related Experiment Videos

  • Analysis of Graves' disease patient serum antibodies (intact IgG and Fab fragments) for TSH receptor binding inhibition and competition assays.
  • Immunoprecipitation studies.

    • Main Results:

    • The TSH receptor is an amphiphilic glycoprotein (approx. 200,000 Da, pI ~5) with a disulphide bridge essential for TSH binding.
    • Graves' disease antibodies directly interact with the TSH receptor, mediating both TSH agonist and antagonist effects.
    • TSH and Graves' IgG/Fab fragments bind to the same or closely related sites on the TSH receptor, and their binding is mutually exclusive.

    Conclusions:

    • The structural integrity of the TSH receptor, particularly its disulphide bridge, is crucial for TSH binding.
    • The interaction of Graves' antibodies with the TSH receptor explains the clinical manifestations of the disease.
    • Evidence strongly suggests that the binding sites for TSH and TSH receptor antibodies on the receptor are highly similar or identical.