Comparison of an ELISA technique with quantal micro-neutralization test for serotyping of HSV-1 or HSV-2-infected...

An enzyme-linked immunosorbent assay (ELISA) using semi-purified herpes simplex antigens extracted from cell nuclei was employed for typing of 32 sera collected from patients infected with HSV-1 or HSV-2. The results were in agreement with those obtained by the quantal micro-neutralization test. Moreover, sera with antibodies which could not be detected by micro-neutralization test could be typed by the ELISA technique according to the HSV isolates.