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Condensed DNA structures derived from bacteriophage heads.

V Virrankoski-Castrodeza, M J Fraser, J H Parish

    The Journal of General Virology
    |January 1, 1982
    PubMed
    Summary
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    Bacteriophage lambda particles release condensed structures containing DNA when osmotically shocked. Protein treatment of these structures releases free DNA, suggesting a supercoiled, possibly nucleosome-like, arrangement within phage heads.

    Area of Science:

    • Molecular Biology
    • Virology
    • Biophysics

    Background:

    • Bacteriophage lambda is a virus that infects bacteria.
    • Understanding the packaging of viral DNA within phage heads is crucial for comprehending viral replication and gene delivery.
    • Previous studies indicated bacteriophage DNA possesses a supercoiled topology.

    Purpose of the Study:

    • To investigate the structural organization of DNA within bacteriophage lambda particles.
    • To determine the mechanism of DNA release from phage heads.
    • To explore the potential for nucleosome-like structures in viral DNA packaging.

    Main Methods:

    • Osmotic shock treatment of bacteriophage lambda particles.
    • Electron microscopy for visualizing released structures.

    Related Experiment Videos

  • Treatment with proteins (cytochrome c) to induce DNA release.
  • Chemical modification using nitrogen mustard to assess DNA integrity.

    • Main Results:

    • Osmotic shock released condensed structures from phage heads.
    • Protein treatment of released structures resulted in free, relaxed DNA.
    • Nitrogen mustard pretreatment led to DNA release in small supercoiled domains.
    • Findings support a supercoiled DNA topology within phage heads, potentially organized in small, nucleosome-like domains.

    Conclusions:

    • Bacteriophage lambda DNA is packaged in a condensed, supercoiled form.
    • The arrangement may involve small, coiled domains analogous to nucleosomes.
    • This conformation could facilitate efficient DNA packaging and release mechanisms.