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Asynchronous bidirectional replication of polyoma virus DNA.

A J Buckler-White, M R Krauss, V Pigiet

    Journal of Virology
    |September 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

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    Polyoma virus DNA replication begins at a single origin (ori). Replicative intermediates show mostly unidirectional synthesis early, and later, two forks moving asynchronously from the origin.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Polyoma virus is a model system for studying eukaryotic DNA replication.
    • Understanding viral DNA replication provides insights into cellular DNA replication mechanisms.

    Purpose of the Study:

    • To analyze the structure of polyoma virus replicative intermediates.
    • To elucidate the mechanism of polyoma virus DNA replication initiation and progression.

    Main Methods:

    • Two-dimensional agarose gel electrophoresis for analyzing DNA structures.
    • Quantitative electron microscopy for visualizing viral DNA intermediates.

    Main Results:

    • DNA replication initiated at a single origin (ori) in nearly all replicative intermediates.

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  • Early intermediates showed predominantly unidirectional synthesis.
  • Mid- and late-stage intermediates featured two replication forks with unequal progression from the origin.

    • Conclusions:

    • Polyoma virus DNA replication initiates unidirectionally from a single origin.
    • Asynchronous fork progression explains the observed structures of replicative intermediates.