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New method for detecting cellular transforming genes.

D G Blair, C S Cooper, M K Oskarsson

Science (New York, N.Y.)

|December 10, 1982

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Tumor induction in nude mice effectively detects transforming genes. This method identifies oncogenes in human cell DNA, enabling tumor formation and further genetic analysis.

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Identifying transforming genes is crucial for understanding cancer.
Current methods for detecting oncogenes can be complex and time-consuming.

Purpose of the Study:

To establish a novel method for detecting dominant transforming genes using tumor induction in mice.
To evaluate the efficacy of using athymic nude mice for oncogene detection.

Main Methods:

Transfection of NIH 3T3 mouse cells with viral and cellular DNA, including DNA from human tumor cell lines.
Injection of transfected cells into athymic nude mice (nu/nu) to induce tumor formation.
Analysis of induced tumors for the presence of human DNA sequences and their transforming potential.

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Main Results:

Cells transfected with viral DNA and DNA from human tumor cell lines induced tumors in nude mice.
Induced tumors contained human DNA sequences.
DNA from these tumors retained the ability to induce tumors and foci upon secondary transfection, confirming the presence of oncogenes.

Conclusions:

Tumor induction in athymic nude mice is a viable and effective method for detecting dominant transforming genes.
This technique facilitates the identification and selection of cellular oncogenes from various sources, including human DNA.
The method offers a powerful tool for oncogene research and cancer diagnostics.