Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Local cellular immunity in tuberculous pleurisy.

K Shimokata, H Kawachi, H Kishimoto

    The American Review of Respiratory Disease
    |November 1, 1982
    PubMed
    Summary

    Tuberculous pleurisy patients show higher T-lymphocyte counts in pleural fluid than blood. Lymphocytes from pleural fluid, unlike those in peripheral blood, effectively produce immune interferon in response to purified protein derivative (PPD).

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Semiquantitative CLDN18 expression and clinical outcomes in patients with CLDN18-positive gastric cancer treated with zolbetuximab plus chemotherapy.

    ESMO gastrointestinal oncology·2026
    Same author

    Distinct Involvement of X-Inactivation in Organogenesis.

    Journal of dental research·2026
    Same author

    Gut microbiome-driven modulation of the tumor immune microenvironment optimizes dual checkpoint blockade in advanced non-small-cell lung cancer.

    ESMO open·2026
    Same author

    Impact of the Lung Immune Prognostic Index in non-small-cell lung cancer patients with PD-L1-low/negative tumors receiving chemoimmunotherapy: a real-world multicenter retrospective study.

    ESMO open·2025
    Same author

    Lenvatinib for patients with previously treated advanced thymic carcinoma in real-world settings.

    ESMO open·2025
    Same author

    Risk factors for and prognostic impact of lateral pelvic lymph node metastasis in patients with rectal neuroendocrine tumors: a single-center retrospective analysis of 214 cases with radical resection.

    Surgery today·2024

    Area of Science:

    • Immunology
    • Infectious Diseases
    • Pulmonary Medicine

    Background:

    • Tuberculous pleurisy is an inflammatory condition affecting the pleural space.
    • Understanding the local immune response in the pleural fluid is crucial for diagnosis and treatment.
    • Lymphocyte function in the pleural space versus peripheral circulation requires further elucidation.

    Purpose of the Study:

    • To compare the characteristics and function of lymphocytes in pleural exudate and peripheral blood of patients with tuberculous pleurisy.
    • To assess the in vitro immune response of these lymphocytes to purified protein derivative (PPD).
    • To investigate the correlation between lymphocyte interferon production and tuberculin skin reaction.

    Main Methods:

    • Studied lymphocytes from pleural exudate and peripheral blood of 18 tuberculous pleurisy patients.

    Related Experiment Videos

  • Quantified T-lymphocyte populations in both sample types.
  • Performed lymphocyte co-culture assays with PPD to measure immune interferon production.
  • Correlated interferon titers with tuberculin skin reaction results.

    • Main Results:

    • Pleural fluid T-lymphocyte counts were significantly higher than peripheral blood counts (p < 0.05).
    • Lymphocytes from pleural fluid demonstrated a robust immune interferon response to PPD (17/18 patients, >128 units/ml).
    • Peripheral blood lymphocytes showed a limited response to PPD (4/18 patients, 4-8 units/ml).
    • Stronger tuberculin skin reactions correlated with higher pleural fluid interferon production (p < 0.05).

    Conclusions:

    • Exudative-sensitized lymphocytes at the site of infection (pleural fluid) are more responsive to specific antigens like PPD than circulating lymphocytes.
    • Pleural fluid lymphocytes exhibit enhanced lymphokine production, indicating a localized and effective immune response in tuberculous pleurisy.
    • These findings highlight the importance of local immune surveillance in the pleural space for combating tuberculosis infection.