Correlation between the antigen-binding activity and the relative concentration of IgE antibodies to mite

Antigen-binding activity of IgE antibodies to mite was determined by the triple antibody assay. Relative concentration of IgE antibodies in mite was determined by RAST. The antigen-binding activity of IgE antibodies correlated with the relative concentration of the antibodies (r equal to 902). This suggests that the absolute concentration of IgE antibodies may be determined by RAST using a reference serum of known absolute concentration of IgE antibodies.