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Related Experiment Videos

Radioimmunoassays for cannabinoids.

C E Cook, H H Seltzman, V H Schindler

    NIDA Research Monograph
    |January 1, 1982
    PubMed
    Summary
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    Radioimmunoassay (RIA) can detect delta-9-tetrahydrocannabinol (THC) in biological samples. New immunogen designs using amyl side chains improve RIA specificity for THC and its metabolites, enhancing accuracy in drug testing.

    Area of Science:

    • Analytical Chemistry
    • Immunology
    • Pharmacology

    Background:

    • Radioimmunoassay (RIA) is a sensitive method for detecting delta-9-tetrahydrocannabinol (THC).
    • Metabolites of THC can interfere with RIA accuracy.
    • Immunogen design is crucial for enhancing assay specificity.

    Purpose of the Study:

    • To develop novel immunogens for improved RIA specificity of delta-9-THC and its metabolites.
    • To utilize the amyl side chain for linking cannabinoids to proteins.
    • To create selective assays for THC and its primary metabolite in biological fluids.

    Main Methods:

    • Cannabinoid compounds (5'-carboxy-delta-8-THC, 5'-carboxy-delta-9-THC) were linked to bovine serum albumin.
    • Rabbits were immunized with these conjugates to generate antisera.

    Related Experiment Videos

  • Radioligands (tritium and iodine-125 labeled) were used for assay development.

    • Main Results:

    • Antisera showed high selectivity for delta-9-THC over its carboxylic acid metabolite.
    • Sensitivity for delta-9-THC in plasma was approximately 2.5 ng/ml using tritium-labeled material.
    • A separate immunogen generated an antiserum highly specific for 11-nor-9-carboxy-delta-9-THC.

    Conclusions:

    • Amyl side chain modification of immunogens effectively enhances RIA specificity for THC and its metabolites.
    • Developed assays provide accurate and specific quantification of delta-9-THC and 11-nor-9-carboxy-delta-9-THC in plasma.
    • These methods offer improved tools for forensic and clinical analysis of cannabis use.