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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
07:23

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Published on: September 5, 2013

Fluorescein diacetate staining of Anaplasma marginale as a possible measure of viability

E E Erp, M Ristic, C A Carson

    American Journal of Veterinary Research
    |February 1, 1978
    PubMed

    Abstract:

    Fluorescein diacetate viability-staining technique was applied to Anaplasma marginale. Marginal bodies took up the stain and became fluorescent. When infected red blood cells were incubated at 55 C, the number of fluorescent Anaplasma decreased at a rate similar to the known loss of viability.

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