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[Retrovirus activating potential of nuclear polyhedrosis viruses in mammalian cell cultures]

J Schmidt, V Erfle

    Zentralblatt Fur Bakteriologie, Mikrobiologie Und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie = International Journal of Microbiology and Hygiene. A, Medical Microbiology, Infectious
    |September 1, 1982
    PubMed

    Insights

    Nuclear polyhedrosis viruses (NPV) do not activate endogenous retroviruses in mammalian cells. This study found NPVs safe for pest control, showing no retrovirus activation or adverse effects on mammal cells.

    Area of Science:

    • Virology
    • Cell Biology
    • Mammalian Cell Culture

    Background:

    • Endogenous retroviruses can be activated by various agents.
    • Nuclear polyhedrosis viruses (NPVs) are used in insect pest control.
    • Potential interaction between NPVs and mammalian retroviruses requires investigation.

    Purpose of the Study:

    • To investigate the potential of nuclear polyhedrosis viruses (NPVs) to activate endogenous C-type retroviruses in mammalian cells.
    • To assess the safety of NPVs for mammalian systems in the context of retroviral activation.

    Main Methods:

    • In vitro cell culture experiments using mouse, rat, monkey, and human cells.
    • Treatment with NPVs, NPV-DNA, and known C-type virus-activating chemicals.
    • Analysis of C-type retrovirus activation and effects on mammalian cell morphology and growth.

    Main Results:

    • C-type retroviruses were activated only by a chemical analogue, not by NPVs or NPV-DNA.
    • NPVs did not potentiate chemical activation of retroviruses in co-treatment experiments.
    • NPV virions maintained infectivity, and no adverse effects on mammalian cells were observed.

    Conclusions:

    • Nuclear polyhedrosis viruses (NPVs) do not activate endogenous C-type retroviruses in mammalian cells.
    • NPVs are unlikely to pose a retroviral risk in mammalian systems.
    • NPV application for pest control is considered safe regarding retroviral activation potential.

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