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Induction and abrogation of suppressor cell function in humans: effect on B cell activation by different polyclonal
Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology
|October 1, 1982
Summary
Concanavalin A (Con-A)-induced suppressor cells inhibit B lymphocyte differentiation into plaque-forming cells (PFCs). This suppression affects various activators, with IgA-secreting cells showing less susceptibility.
Area of Science:
- Immunology
- Cell Biology
Background:
- Polyclonal B lymphocyte activation is crucial for immune responses.
- Suppressor cells can modulate immune cell differentiation.
Purpose of the Study:
- To investigate the impact of concanavalin A (Con-A)-induced suppressor cells on B lymphocyte differentiation into plaque-forming cells (PFCs).
- To determine the susceptibility of different B lymphocyte populations and immunoglobulin isotypes to suppression.
Main Methods:
- Utilized a reverse plaque-forming cell (PFC) assay.
- Studied the effect of Con-A-pretreated suppressor cells on B lymphocytes activated by pokeweed mitogen (PWM), Staphylococcus aureus, and Epstein-Barr virus (EBV).
- Assessed the radiosensitivity of suppressor cells.
Main Results:
- Con-A-induced suppressor cells inhibited PFC differentiation across all tested polyclonal activators.
- Epstein-Barr virus (EBV)-stimulated B cells exhibited the lowest PFC response and were least sensitive to suppression.
- IgA-secreting cells were less suppressible than IgM- and IgG-secreting cells.
- Suppressor cells demonstrated radiosensitivity.
Conclusions:
- Concanavalin A-induced suppressor cells effectively inhibit polyclonal B lymphocyte activation and differentiation.
- The degree of suppression varies depending on the B cell activator and the immunoglobulin isotype produced.
- Radiosensitivity suggests a cellular mechanism for suppression.