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Murine retrovirus genome directs the synthesis of gag protein precursor early after infection
Biochimie
|October 1, 1982
Abstract:
Incoming type C retroviral genomic 35S RNA is present in polysomes of undifferentiated and differentiated murine teratocarcinoma cell lines at 4 hours after infection. At the same time a 65,000 daltons viral specific protein is produced by the infected cells. These data present evidence that incoming viral RNA serves as messenger for the synthesis of gag protein precursor Pr65 early in the infectious cycle of ecotropic murine retrovirus.
Insights
Newly identified retroviral genomic RNA acts as a messenger in infected cells. This viral RNA directs the synthesis of a key gag protein precursor early in the retroviral infectious cycle.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Murine teratocarcinoma cell lines are utilized as a model system.
- Type C retroviruses are studied for their replication mechanisms.
Purpose of the Study:
- To investigate the role of incoming retroviral RNA in protein synthesis.
- To determine the timing of viral protein production during infection.
Main Methods:
- Infection of murine teratocarcinoma cells with type C retrovirus.
- Analysis of polysomal RNA.
- Detection of viral-specific proteins.
Main Results:
- Genomic 35S RNA of type C retrovirus was found in polysomes of infected cells.
- A 65,000-dalton viral-specific protein was produced 4 hours post-infection.
- Evidence suggests incoming viral RNA functions as mRNA.
Conclusions:
- Incoming viral RNA serves as the messenger for gag protein precursor (Pr65) synthesis.
- This process occurs early in the infectious cycle of ecotropic murine retrovirus.