Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hotting-up the complement-fixation test.

C J Ronalds, A E Hardiman, P D Griffiths

    The Journal of Hygiene
    |February 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Change in diagnostic confidence brought about by using in utero MRI for fetal structural brain pathology: analysis of the MERIDIAN cohort.

    Clinical radiology·2017
    Same author

    Making dollars and sense out of a screening programme.

    Reviews in medical virology·2017
    Same author

    The assessment of fetal brain growth in diabetic pregnancy using in utero magnetic resonance imaging.

    Clinical radiology·2017
    Same author

    Medical practice driven by legislators rather than by regulators.

    Reviews in medical virology·2016
    Same author

    Compartmentalized dynamics of cytomegalovirus replication in treated congenital infection.

    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2016
    Same author

    Demonstration of Normal and Abnormal Fetal Brains Using 3D Printing from In Utero MR Imaging Data.

    AJNR. American journal of neuroradiology·2016
    Same journal

    Quantitative aspects of antigen-antibody reactions; a theory and its corollaries.

    The Journal of hygiene·2010
    Same journal

    A.L.-63, the original British Army louse powder.

    The Journal of hygiene·2010
    Same journal

    A routine method of bacteriological analysis and grading of ice-creams; with records of eleven years' application.

    The Journal of hygiene·2010
    Same journal

    Three rapid tests for the estimation of tropical fitness of fabrics.

    The Journal of hygiene·2010
    Same journal

    Enteric fevers in Egypt.

    The Journal of hygiene·2010
    Same journal

    Fluorine alopecia.

    The Journal of hygiene·2010
    See all related articles

    Optimizing the complement-fixation (CF) test for viral diagnostics is crucial. Increasing incubation temperature to 15°C significantly enhances sensitivity for detecting IgG antibodies, improving viral diagnosis.

    Area of Science:

    • Immunology
    • Virology
    • Diagnostic Assays

    Background:

    • The complement-fixation (CF) test is a widely used serological assay for detecting antibodies.
    • Optimizing incubation conditions is essential for maximizing the sensitivity and accuracy of the CF test.
    • Previous studies have utilized varying incubation temperatures, but a definitive optimal temperature for enhanced sensitivity remains to be established.

    Purpose of the Study:

    • To investigate the effect of modifying incubation temperature on the sensitivity of the complement-fixation (CF) test.
    • To determine the optimal incubation temperature for the CF test to achieve maximum sensitivity in detecting viral IgG antibodies.
    • To evaluate the impact of increased incubation temperature on false-positive reactions and anticomplementary activity.

    Main Methods:

    Related Experiment Videos

    • The study involved a detailed investigation of the complement-fixation (CF) test.
    • Incubation temperatures were systematically modified to assess their effect on test sensitivity.
    • Antibody detection was performed using CF, with results compared against ultrasensitive radioimmunoassay procedures for IgG antibodies.

    Main Results:

    • Increasing incubation temperature progressively enhanced CF test sensitivity, reaching a maximum at 15°C.
    • At 15°C, the geometric mean titre of seropositive samples was significantly greater than at 4°C for varicella-zoster virus, cytomegalovirus, and rubella virus.
    • All sera containing IgG antibodies, as confirmed by radioimmunoassay, were detected by CF at 15°C without false-positive reactions.

    Conclusions:

    • The complement-fixation (CF) test should be performed at 15°C to achieve optimum sensitivity for viral antibody detection.
    • This higher incubation temperature (15°C) allows for the detection of IgG antibodies against various viruses, including varicella-zoster virus, cytomegalovirus, and rubella virus.
    • The ability to detect significant rises in antibody titre was not compromised at the optimized higher incubation temperature.