Adrenal maturation of the sheep fetus during late pregnancy

The mechanism by which adrenal growth is achieved and steroidogenesis is stimulated in the fetal lamb during late pregnancy has been studied. Fetal plasma ACTH levels remained roughly constant between 14 and 3 days pre partum (298 +/- 38 pg/ml), then increased to a mean of 634 +/- 89 pg/ml. The rise in ACTH did not occur before the pre partum rise in cortisol. The increase in adrenal weight was achieved in three periods, one of DNA duplication between two phases of cellular hypertrophy. Cellular hypertrophy was accompanied by an important biosynthesis of membrane proteins. This development might partly explain the increased production rate of cortisol by the fetal adrenals during late pregnancy. At the same time, the number of ACTH receptors per 2 adrenals increased 5-fold. There was a close correlation between the levels of fetal plasma corticosteroids and the number of ACTH receptors during late pregnancy. It has been suggested that receptor modulation is one of the factors responsible for the enhanced sensitivity of the fetal adrenals to ACTH just before parturition. Preliminary results on the endocrine regulation of that development are also presented.