Related Experiment Videos
On the terminally redundant sequences of bacteriophage T3 DNA
Virology
|January 30, 1983
Abstract:
The nucleotide sequences of the termini of mature DNA from T3 phage were determined. A directly repeated sequence of 230 base pairs, corresponding to the terminal redundancy of T3 DNA, was found in the left and right end of the genome. The sequence of the terminal regions of T3 DNA was compared with that of T7 DNA. There are discrete sequences with significant homology, specifically in the regions around the two ends of the terminally redundant sequences. 5'-CCTAAAG and its variants appear frequently in both ends of the genome. T3 DNA fragments carrying the terminally redundant sequences were isolated from concatemeric DNA and analyzed by restriction enzymes.