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Updated: Jul 12, 2026

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
Published on: November 9, 2017
Cell-type-specific actin mRNA populations in dictyostelium discoideum
Abstract:
We have analyzed actin mRNA sequences present in the terminal stages of the development of Dictyostelium discoideum. Four different actin mRNA sequences were detected in migrating pseudoplasmodia. Nucleotide sequence analysis of primer-extension products derived from the four mRNA sequences showed that they each encoded an actin protein with the same eight N-terminal amino acids and that they did not derive from transcription of any previously characterized actin gene. Preculmination pseudoplasmodia of Dictyostelium contain two distinct populations of committed cells, termed prespore and prestalk cells. We show that prestalk cells contain all four of the actin mRNA sequences found in pseudoplasmodia, while prespore cells contain only three of the sequences, and mature spores contain only two. Thus there is a differential loss of actin mRNA sequences during spore-cell differentiation in Dictyostelium.
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