Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Benzo[a]pyrene-collagen interactions.

E Fujimori, D V Crabtree

    Chemico-Biological Interactions
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Benzo[a]pyrene (BaP) binds to collagen, inhibiting fibril formation. This interaction, particularly with collagen telopeptides, is further affected by UV light, impacting collagen structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Changes in nutritional status of pregnant women assisted at the basic health network, Santo Andre, Brazil].

    Revista latino-americana de enfermagem·2002
    Same author

    Relative effectiveness of iron bis-glycinate chelate (Ferrochel) and ferrous sulfate in the control of iron deficiency in pregnant women.

    Archivos latinoamericanos de nutricion·2001
    Same author

    Tubulins in the primate retina: evidence that xanthophylls may be endogenous ligands for the paclitaxel-binding site.

    Bioorganic & medicinal chemistry·2001
    Same author

    [Rice and bean diets increase hepatic and plasmatic activity of gammaglutamyltranspeptidase in young rats].

    Archivos latinoamericanos de nutricion·2001
    Same author

    [Iron nutritional status in pregnant adolescents, São Paulo, Brazil].

    Archivos latinoamericanos de nutricion·1999
    Same author

    DL-methionine supplementation of rice-and-bean diets affects gamma-glutamyltranspeptidase activity and glutathione content in livers of growing rats.

    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·1999
    Same journal

    Integrated pathways of T-2 toxin-induced neurotoxicity and protection by sodium butyrate in quails.

    Chemico-biological interactions·2026
    Same journal

    Chlorinated Bis-phenolic Disinfectants Inhibit Human and Rat Aromatase.

    Chemico-biological interactions·2026
    Same journal

    Integrative in silico and in vitro Discovery of a Novel Thiazolidinone Derivative as Arginase Inhibitor with Potent Antileishmanial Activity.

    Chemico-biological interactions·2026
    Same journal

    Sex- and size-dependent impacts of tire wear particles and zinc oxide nanoparticles on adult zebrafish: integrated evidence from physiology, gut microbiota networks, and hepatic transcriptomics.

    Chemico-biological interactions·2026
    Same journal

    AMPK activation by AICAR mitigates hypoxic corneal damage through rebuilding energy and metabolic homeostasis.

    Chemico-biological interactions·2026
    Same journal

    Interactions-guided blueprint of acetylcholinesterase binding: A review on structural and molecular determinants.

    Chemico-biological interactions·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Materials Science
    • Toxicology

    Background:

    • Type I collagen is crucial for tissue structure and fibrillogenesis.
    • Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon with known toxicological effects.
    • Understanding pollutant interactions with biomolecules is vital for toxicology and environmental health.

    Purpose of the Study:

    • To investigate the in vitro interactions between benzo[a]pyrene (BaP) and type I collagen.
    • To determine how BaP affects collagen fibril formation and structural integrity.
    • To explore the influence of UV irradiation on BaP-collagen complexes.

    Main Methods:

    • Spectroscopic analysis (fluorescence) to monitor BaP-collagen binding.
    • In vitro fibril formation assays.

    Related Experiment Videos

  • Viscosity measurements and Circular Dichroism (CD) spectroscopy to assess collagen conformation.
  • Thermal denaturation studies to probe structural changes.

    • Main Results:

    • BaP fluorescence increases upon binding to collagen.
    • Bound BaP significantly inhibits collagen fibril formation.
    • UV irradiation of BaP-collagen complexes enhances inhibition of fibril formation.
    • Collagen's helical structure and size remain unaltered by BaP or UV light.
    • BaP fluorescence changes during collagen thermal denaturation, indicating interaction with specific regions.

    Conclusions:

    • BaP interacts with type I collagen, primarily affecting the hydrophobic telopeptide regions.
    • These interactions inhibit collagen fibrillogenesis, a process critical for tissue integrity.
    • UV-induced photooxidation of BaP exacerbates its inhibitory effect on fibril formation.
    • The telopeptide region, essential for fibril assembly, is a key site of BaP interaction.