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Applications of the macrophage electrophoretic mobility test (M.E.M.)
Abstract:
This paper describes the principle of the M.E.M. test (macrophage electrophoretic mobility). Human lymphocytes from normal or diseased patients, if exposed to an antigen to which they were sensitized may produce a factor which would reduce the electrophoretic mobility of a normal guinea pig peritoneal macrophage population. It is shown a summary of the applications of the test system and disadvantages of using P.E.C. and a summary of test system used for assessing slowing factors produced by lymphocyte-antigen reaction.
Insights
The macrophage electrophoretic mobility (M.E.M.) test detects factors from sensitized lymphocytes that alter macrophage movement. This immune response assay has various applications but also noted disadvantages.
Area of Science:
- Immunology
- Cellular immunology
Background:
- The macrophage electrophoretic mobility (M.E.M.) test is a method for detecting immune responses.
- Lymphocytes from sensitized individuals can produce factors affecting macrophage behavior.
Purpose of the Study:
- To describe the principle of the M.E.M. test.
- To summarize applications and disadvantages of the M.E.M. test system.
- To review systems for assessing lymphocyte-antigen reactions.
Main Methods:
- Utilizes human lymphocytes and guinea pig peritoneal macrophages.
- Involves exposing lymphocytes to specific antigens.
- Measures changes in macrophage electrophoretic mobility.
Main Results:
- Sensitized lymphocytes produce factors that reduce macrophage electrophoretic mobility.
- The M.E.M. test can detect these lymphocyte-derived factors.
- Identified applications and limitations of the test system.
Conclusions:
- The M.E.M. test is a viable method for assessing cellular immune responses.
- Understanding the factors influencing macrophage mobility is crucial.
- Further refinement of the test system is warranted for broader applications.