Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calmodulin increases in selective brain regions with opioid dependence.

K A Bonnet, L Engelberg, S A Gusik

    Life Sciences
    |November 15, 1982
    PubMed
    Summary

    Opioid agonists disrupt calcium in thalamic membranes, inhibiting adenylate cyclase. Chronic morphine use increases calmodulin in the thalamus, suggesting molecular adaptations to opioid exposure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A parametric study of the N40 auditory evoked response in rats.

    Biological psychiatry·1997
    Same author

    Asperger syndrome in neurologic perspective.

    Journal of child neurology·1996
    Same author

    Correlates of vascular access occlusion in hemodialysis.

    American journal of kidney diseases : the official journal of the National Kidney Foundation·1994
    Same author

    Prealbumin and lipoprotein(a) in hemodialysis: relationships with patient and vascular access survival.

    American journal of kidney diseases : the official journal of the National Kidney Foundation·1993
    Same author

    Evaluation of a nisin-based germicidal formulation on teat skin of live cows.

    Journal of dairy science·1992
    Same author

    The effect of hyperthyroidism on opiate receptor binding and pain sensitivity.

    Life sciences·1990

    Area of Science:

    • Neuropharmacology
    • Molecular Biology
    • Cellular Signaling

    Background:

    • Opioid agonists interact with cellular mechanisms in the brain.
    • Calcium ions play a critical role in signal transduction pathways.
    • Adenylate cyclase activity is a key indicator of cellular response to stimuli.

    Purpose of the Study:

    • To investigate the acute effects of opioid agonists on thalamic membranes.
    • To examine the long-term molecular adaptations in brain regions following chronic morphine administration.
    • To determine the role of calmodulin in mediating these changes.

    Main Methods:

    • Acute challenge of thalamic membranes with opioid agonists.
    • Measurement of calcium displacement and adenylate cyclase stimulation.
    • Chronic morphine administration (3 days and 3 weeks).
    • Quantification of calmodulin levels in various brain regions.

    Main Results:

    • Opioid agonists acutely displaced calcium and inhibited isoproterenol-stimulated adenylate cyclase in thalamic membranes.
    • Chronic morphine administration led to significant calmodulin level increases in thalamic membranes.
    • Calmodulin levels remained unchanged in other brain regions including periaqueductal gray, striatum, amygdala, and hypothalamus.

    Conclusions:

    • Acute opioid receptor activation affects calcium homeostasis and adenylate cyclase signaling in the thalamus.
    • Chronic morphine exposure induces region-specific upregulation of calmodulin in the thalamus.
    • Calmodulin may be involved in the adaptive mechanisms of the thalamus to prolonged opioid treatment.

    Related Experiment Videos