Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ScrFI: a new sequence-specific endonuclease from Streptococcus cremoris.

G F Fitzgerlad, C Daly, L R Brown

    Nucleic Acids Research
    |December 20, 1982
    PubMed
    Summary

    Researchers discovered a new enzyme, ScrFI, from Streptococcus cremoris F. This novel sequence-specific endonuclease is the first identified from lactic streptococci used in dairy fermentations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Upper gastrointestinal training in the UK and Ireland: a Roux Group Study.

    Annals of the Royal College of Surgeons of England·2024
    Same author

    Temporal effects on death by suicide: empirical evidence and possible molecular correlates.

    Discover mental health·2023
    Same author

    Biocompatibility of Antifogging SiO-doped Diamond-Like Carbon Laparoscope Coatings.

    Applied surface science·2023
    Same author

    Rhythm vs rate control strategy effect on direct current cardioversion outcomes for atrial fibrillation.

    Irish medical journal·2023
    Same author

    Causes of widespread foliar damage from the June 2021 Pacific Northwest Heat Dome: more heat than drought.

    Tree physiology·2023
    Same author

    Cardiac sarcoidosis with complete atrioventricular block.

    QJM : monthly journal of the Association of Physicians·2022

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Enzymology

    Background:

    • Lactic streptococci are crucial microorganisms in dairy fermentations.
    • Endonucleases play vital roles in DNA metabolism and biotechnology.
    • Limited characterized enzymes exist from dairy-relevant bacterial species.

    Purpose of the Study:

    • To isolate and characterize a novel sequence-specific endonuclease from Streptococcus cremoris F.
    • To determine the DNA recognition sequence and cleavage site of the novel enzyme.
    • To establish the significance of this enzyme within the context of lactic streptococci.

    Main Methods:

    • Isolation and purification of the endonuclease from Streptococcus cremoris F.
    • DNA substrate preparation for enzyme activity assays.
    • Determination of the specific DNA recognition sequence and cleavage pattern.

    Main Results:

    • A novel sequence-specific endonuclease, designated ScrFI, was successfully isolated.
    • ScrFI recognizes and cleaves a specific DNA sequence (details omitted for brevity).
    • This represents the first endonuclease reported from lactic streptococci used in dairy fermentations.

    Conclusions:

    • The discovery of ScrFI expands the repertoire of available restriction enzymes.
    • This enzyme offers potential applications in molecular biology and biotechnology.
    • Its identification highlights the untapped enzymatic potential within dairy-associated microorganisms.

    Related Experiment Videos