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Related Experiment Videos

Cell transformation by simian virus 40 mutants.

R Monier

    Princess Takamatsu Symposia
    |January 1, 1982
    PubMed
    Summary

    The simian virus 40 (SV40) early region

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    Area of Science:

    • Virology
    • Cell Biology
    • Cancer Research

    Background:

    • The early region of simian virus 40 (SV40) is implicated in cellular transformation.
    • Understanding SV40's role in transformation is crucial for cancer research.

    Purpose of the Study:

    • To investigate the role of the SV40 early region in cellular transformation.
    • To assess the requirement of the A gene for cell line conversion.
    • To evaluate SV40 mutants in conjunction with tumor promoters for inducing anchorage-independent growth.

    Main Methods:

    • Constructed double mutants of SV40 (d1-54-59 with tsA or 3'-terminal A gene deletion).
    • Selected transformants by cloning infected mouse or rat embryo cells at low density.
    • Tested anchorage-independent growth and soft-agar colony formation in Swiss 3T3 cells with tumor promoters like TPA.

    Main Results:

    • Permanent expression of the SV40 A gene is necessary for converting mouse or rat embryo cells into continuous cell lines.
    • SV40-tsA-infected cells formed macrocolonies in soft-agar at restrictive temperatures with TPA.
    • A correlation was observed between macrocolony formation and the promoting activity of compounds like teleocidin B and anthralin.

    Conclusions:

    • The SV40 A gene is essential for sustained cellular transformation.
    • SV40-tsA-infected Swiss 3T3 cells provide a sensitive system for detecting tumor promoter activity.
    • This system offers a rapid and straightforward method for screening potential tumor promoters.

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