Improved immunofluorescence techniques with microplates for the detection of M and G immunoglobulins against...

A selection of microplates and an inverted microscope were used for the detection of immuno-fluorescence in large numbers of infected cell monolayers. The optical features of this instrument in combination with either Terasaki or Microlymp plates, simplify procedures and allow economical use of reagents, for determining fluorescent antibody levels. The techniques described were applied for estimating M and G immunoglobulins against rotavirus in elderly patients and the validity of the tests was established.