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Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
Published on: March 2, 2014
Mouse immunoglobulin antibodies require complement for neutralization of mouse retroviruses
Journal of Virology
|March 1, 1983
Summary
Guinea pig complement significantly boosted mouse antibody neutralization of endogenous ecotropic murine leukemia viruses. This complement-dependent activity, primarily from immunoglobulin M, was specific to Akv-2 induced viruses.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- Murine leukemia viruses (MLVs) are retroviruses that can infect mice.
- Antibodies play a crucial role in neutralizing viral infections.
- Complement system activation can enhance antibody-mediated immune responses.
Purpose of the Study:
- To investigate the role of guinea pig complement in enhancing the neutralizing capacity of mouse antibodies against endogenous ecotropic MLVs.
- To characterize the specificity and immunoglobulin class of these complement-dependent antibodies.
Main Methods:
- Testing mouse immune sera for neutralizing capacity against ecotropic MLVs with and without guinea pig complement.
- Characterizing antibody specificity using different MLV strains (Friend, Moloney).
- Identifying the immunoglobulin fraction responsible for complement-dependent neutralization.
Main Results:
- Guinea pig complement significantly enhanced the neutralizing activity of mouse antibodies against endogenous ecotropic MLVs.
- Mouse antibodies showed low neutralizing capacity without complement.
- High-titer, type-specific antibodies were found in NFS congenic mice with the Akv-2 locus.
- These antibodies did not neutralize Friend or Moloney MLVs.
- The complement-dependent antibody activity was attributed to immunoglobulin M.
Conclusions:
- Guinea pig complement potentiates mouse antibody neutralization of endogenous ecotropic MLVs.
- Immunoglobulin M antibodies are key mediators of this complement-dependent neutralization.
- The observed antibodies are specific for Akv-2 induced MLVs, highlighting viral-specific immune responses.
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