Partial purification of renal lysosomal substances producing angionecrosis and increased vascular permeability

The lysosomal contents of hog kidney cortex, which induce angionecrosis and/or an increase of vascular permeability, were obtained by differential centrifugation and osmotic shock treatment, and were purified by various chromatographic techniques and electrophoresis. The material producing an increase in vascular permeability of the rabbit skin was purified 600-fold over the homogenate; however, it showed practically no angionecrotic activity. Another substance producing angionecrosis without pressor activity was partially purified.