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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
Published on: August 5, 2011
Expression of transferrin receptors during erythroid maturation
Experimental Cell Research
|April 1, 1983
Summary
A new monoclonal antibody, F111/2Dl, identifies transferrin receptors on cells. This antibody was used to study transferrin receptor distribution during red blood cell development in normal and cancerous cell lines.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Monoclonal antibodies are crucial tools in cell biology and immunology.
- The transferrin receptor plays a vital role in cellular iron uptake and is implicated in various cellular processes, including cell proliferation and differentiation.
- The K562 cell line is a human erythroleukemia cell line commonly used to study erythroid differentiation.
Purpose of the Study:
- To describe a newly produced monoclonal antibody, F111/2Dl.
- To characterize the cell surface determinants recognized by F111/2Dl.
- To investigate the distribution of transferrin receptors during erythroid maturation using F111/2Dl and OKT-9.
Main Methods:
- Production of monoclonal antibody F111/2Dl by immunizing C3H/He mice with the K562 cell line.
- Characterization of F111/2Dl binding to cell surface determinants.
- Comparison of F111/2Dl distribution with OKT-9 (a known transferrin receptor antibody).
- Investigation of transferrin receptor distribution on normal bone marrow, peripheral blood, and K562 cells during in vitro induced erythroid differentiation.
Main Results:
- The F111/2Dl antibody detects cell surface determinants.
- The distribution of determinants recognized by F111/2Dl is similar to those identified by OKT-9.
- OKT-9 is known to identify the transferrin receptor.
- The study investigated transferrin receptor distribution during erythroid maturation in various cell types.
Conclusions:
- F111/2Dl is a monoclonal antibody that recognizes cell surface determinants similar to the transferrin receptor.
- The antibody can be used to study the distribution of transferrin receptors during erythroid maturation.
- This research contributes to understanding iron metabolism and cell differentiation in erythropoiesis.
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