Amino acid sequence around the active site cysteine residue of calcium-activated neutral protease (CANP)

The active site of calcium-activated neutral protease (CANP) was specifically labeled with iodoacetic acid. The active site hepta peptide was isolated from carboxymethylated CANP after digestion with proteases and the sequence was determined. The sequence indicates that CANP is a thiol protease and its comparison with other thiol proteases is described.