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Related Experiment Videos

Simplified method for typing herpes simplex virus by restriction endonuclease analysis.

M Q Arens, E M Swierkosz

    Journal of Clinical Microbiology
    |March 1, 1983
    PubMed
    Summary

    A new simplified method accurately types herpes simplex virus (HSV) without radioactive isotopes. This technique isolates viral DNA from cellular DNA for clear analysis.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Biochemistry

    Background:

    • Herpes simplex virus (HSV) typing is crucial for clinical diagnosis and epidemiological studies.
    • Existing methods for HSV typing can be complex and may involve radioactive isotopes, posing safety and disposal concerns.

    Purpose of the Study:

    • To develop a simplified and unambiguous method for typing herpes simplex virus.
    • To eliminate the need for radioactive isotopes in viral DNA analysis.
    • To ensure viral DNA is free from cellular DNA contamination.

    Main Methods:

    • Cell-associated virus production at 34°C.
    • Separation of cellular and viral DNA using Dounce homogenization and differential centrifugation to remove nuclei.
    • Preparation of viral nucleic acid from the cytoplasmic fraction.
    • Analysis of viral DNA following restriction endonuclease cleavage.

    Main Results:

    • The developed method provides unambiguous typing of herpes simplex virus.
    • The technique successfully isolates viral DNA from cellular DNA.
    • Radioactive isotopes are not required for the procedure.
    • The isolated viral DNA is free from interfering cellular DNA.

    Conclusions:

    • This simplified method offers an efficient and safe approach for herpes simplex virus typing.
    • The technique is suitable for routine laboratory use, improving diagnostic capabilities.
    • The elimination of radioactive isotopes makes the method more accessible and environmentally friendly.

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