The adrenal chromaffin cell as a model to study the co-secretion of enkephalins and catecholamines

The enkephalins, endogenous opioid pentapeptides first discovered in brain, are present in high concentrations in the adrenal medulla chromaffin cell. The enkephalins and other peptides containing enkephalin sequences are stored with catecholamines in the secretory organelles (chromaffin vesicles); these peptides are apparently incorporated into the vesicles at the time of their biosynthesis as opposed to later accumulation, as is the case with catecholamines. The enkephalins, catecholamines and other soluble components of the vesicle are co-secreted by the process of exocytosis. Regulatory mechanisms, apparently triggered by a critical catecholamine pool, control the synthesis of enkephalins. These mechanisms allow for rapid recovery of enkephalin content after secretion. These findings have been extended from the chromaffin cell to the ontogenically related sympathetic neurons and pheochromocytoma tumors. Secreted enkephalins and related peptides reach ubiquitous opiate receptors through the synaptic gap or the circulation and may modulate a number of important systemic functions. The co-storage and co-secretion of adrenomedullary opioid peptides and catecholamines is only one of a growing number of examples of co-existence of multiple messengers in single neuronal or endocrine cell types. Co-secreted multiple messengers may act in a co-ordinated fashion to produce integrated organismal responses.