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Ionic currents in single isolated bullfrog atrial cells.

J R Hume, W Giles

    The Journal of General Physiology
    |February 1, 1983
    PubMed
    Summary
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    Researchers developed a new voltage-clamp technique to measure ionic currents in bullfrog atrial cells. This method identified five distinct ionic currents crucial for action potential generation in these cardiac cells.

    Area of Science:

    • Cardiology
    • Electrophysiology
    • Cell Biology

    Background:

    • Enzymatic dispersion yields quiescent single bullfrog atrial cells with normal electrical properties.
    • Previous studies established baseline electrophysiological characteristics of these cells.

    Purpose of the Study:

    • To refine techniques for quantitative measurement of transmembrane ionic currents.
    • To identify specific ionic current components responsible for action potential phases.

    Main Methods:

    • Development of a novel one-microelectrode voltage-clamp technique using low-resistance micropipettes.
    • Application of voltage-clamp experiments on isolated single atrial cells.

    Main Results:

    • Identification of five distinct ionic currents: transient Na+, TTX-resistant Ca++, persistent inward, outward K+, and background inward rectifier currents.

    Related Experiment Videos

  • Demonstration of a small, TTX-insensitive inward current using initial two-microelectrode experiments.

    • Conclusions:

    • The new one-microelectrode voltage-clamp technique is effective for quantitative ionic current studies in cardiac cells.
    • This method is suitable for studying electrophysiology in small cells with similar properties.